ACUTE TOXICITY AND EFFECT OF MORINGA OLEIFERA LEAF EXTRACT ON BLOOD GLUCOSE LEVEL OF STREPTOZOCIN INDUCED HYPERGLYCEMIA IN ALBINO RATS
Keywords:
Acute, toxicity, Moringa, Blood glucose, Streptozocin, Rats.Abstract
The acute oral toxicity of graded doses of aqueous extract of Moringa oleifera leaves was investigated using the Up and Down procedure. The hypoglycemic effect of the extract was also investigated on streptozocin induced hyperglycaemic rats. The result revealed that the lethal dose of the plant was greater than 5000mg/kg body weight. The administration of graded doses of the extract produced significant (P<0.05) decreases in the blood sugar levels of the treated animals when compared to the control group. The significant hypoglycemic effect may be the justification for M.oleifera folkoloric use as a potential hypoglycemic agent. In conclusion M. oleifera leaf extract may be said to possess hypoglycaemic property.
References
Asatoor, A. M. and Kings, E.J.: Estimation of blood glucose. J. of Biochem., 56:27-31 (1954).
Bruce, R.D.: A confirmatory study of Up and Down method of Acute Oral Toxicity Testing. Furdam. Appl. Toxicol. 8:97-100 (1987).
Bruce, R.D.: An Up and Down procedure for Acute Toxicity Testing. Fundam. Appl. Toxicol. 5:151-157 (1985).
Council for Int. Org. Med. Sci. (CIOMS) 1211 Geneva, 27, Switzerland, c/o W.H.O. (1985).
Dahot, M.U.: Vitamin content of the flowers and seeds of Moringa oleifera. Pak J. Biochem. 21(1-2): 21-24 (1988).
Dennis, V.P.: Mammalian metabolism of xenobiotic chemicals. In: Toxicology and Newborn. Kacew, S. and Reasor, M.J. eds. (Chapman Hall, London 1984).
Dixon, W.J.: The Up-and-Down method for small samples J. Amer. Stat. Assoc. 60: 967-978 (1965).
Dixon, W.J.: Staircase Bioassay. The Up-and-Down method. Neurosci. Biobehav. Rev. 15:47-50 (1991).
Dsouza, J. and Kulkarni, A.R.: Comparative Studies on nutritive values of tender foliage of seedlings and mature plants of Moringa oleifera. J. Econ. Tax. Bot., 17(2): 479-485 (1993).
Folkard, G.K. and Sutherland, J.P.: Moringa oleifera – a multipurpose tree. (Rugby, U. K. 1996.)
Graph Pad Instat. Graphed Instat version 3.0 for windows 95. (graphed software, San Diego California USA www.graphpad. com2003).
Kubo, H., Kobayashi, J., Higashiyama, K., Kamei, J., Fujii, Y. and Ohmiya, S.: Hypoglycemic effects of phenyl-octahydro-quinolizin-2-1 in mice. Institute of medical chemistry, Hoshi University. Tokyo, Japan. Biol. Pharm. Bull. 23(9): 1114-1117 (2000).
Li, M., Ku, W., Wang, Y., Wah, H. and Tian, C.: Hypoglycemic effects of saponins from Tribulus terrestis. J. Nat. Prod. 25(6):420-422 (2002).
Lowell, J.F. and Sreeja, K.V.: Cultivation of Moringa. In: The Miracle tree. The multipurpose attributes of Moringa Edited by Lowell, J.F. (C.T.A., The Netherlands 2001).
Mekonnen, E., Hunde, A. and Damecha, G.: Hypoglycemic effect of Moringa stenopetala acqueous extracts in rabbits. Phyto. Res. 11(2): 147-148 (1997).
Ojo, N.A.: Toxicity and hypoglycemic effect of Moringa oleifera leaf extract in albino rats. Ph.D. Thesis (University of Maiduguri, Maiduguri, Nigeria 2010).
Oladele, S.B., Ayo, J.O. and Adaudi, A.O.: Medicinal and physiological properties of flavonoids, coumarin derivatives and anthraquinones of plant origin. West Africa J. Pharm. Drug Res. 11:134-144 (1995).
Pritchett, K.R. and Corning, B.F.: Biology and Medicine of Rats. In: laboratory Animal Medicine and Management, Reuter, J.D. and Suckow, M.A. (Eds.) (International Veterinary Information Service Ithaca NY. 2004).
Ram, J.: Moringa a highly nutritious vegetable tree. Tropical Rural and Island. A toll Development Experimental Station (TRADES) Tech. Bull. No. 2, 1994).
Ramachandran, C., Peter, K.V. and Gopalakrisshnan, P.K.: Drumstick (Moringa oleifera): A multipurpose Indian vegetable. Econ. Bot. 34 (3): 276-283 (1980).
Sanni, S.: Pharmacological and Toxicological effects of Ocimum basilicum L. water extracts in rats. Ph.D Thesis (Uthman Danfadio University, Sokoto (2007).
Sudheesh, S., Manilal, V.B., Vijayalakshmi, N.R.: Potential health promoting effect of flavonoids. A comparative study on hypolipidemia and hypoglycemic activities. In: Abstract of posterts, final program and book of abstract (53rd Annual Meeting of the Society of Medicinal Plant Research (GA) and Joint Congress with the Italian Society of Phytochemical (SIF), Florence, Italy 2005).
Verma, S.C., Banerji, R., Misra, G. and Nigam, S.K.L.: Nutritional Value of Moringa. Curr. Sci. 45(21):769-77 (1976).
Walum, E Acute oral toxicity. Environ. HHL. Persp. 106: 497-503 (1998).
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